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This product is a cell line stably expressing the CRISPR Cas9 nuclease. This cell line also expresses the hygromycin resistance gene. In combination with separately transfected or transduced single guide RNAs (sgRNAs), this cell line
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Journal: BMJ Open Respiratory Research
Article Title: Differential response to bacteria, and TOLLIP expression, in the human respiratory tract
doi: 10.1136/bmjresp-2014-000046
Figure Lengend Snippet: TOLLIP expression in nasal and alveolar epithelium. (A) T84 cells were plated at two different cell densities: 5×10 5 per well (lanes 1, 2); 2×10 6 , (lanes 3, 4). Lane 5 represents a negative control without the reverse transcriptase. GAPDH was used as a housekeeping gene. (B) TOLLIP expression was quantified in primary nasal and alveolar epithelium. *p<0.05 by Mann-Whitney U test. (C and D) Cell lines were infected with Staphylococcus aureus strain Newman. RNA extraction was performed followed by RT-PCR. Panel C shows RPMI 2650 cells—and panel D A549 cells—infected with S. aureus . Lanes: (1) positive control for TOLLIP from cell line T84; (2 and 3) unstimulated; (4 and 5) cells with S. aureus at 1.1×10 5 cfu/mL; (6 and 7) cells with S. aureus at 1.6×10 5 cfu/mL. GAPDH was used as a housekeeping gene. Band size for TOLLIP 347 bp and for GAPDH 727 bp (TOLLIP, Toll-interacting protein; RT-PCR, reverse transcriptase PCR).
Article Snippet: The human
Techniques: Expressing, Negative Control, Reverse Transcription, MANN-WHITNEY, Infection, RNA Extraction, Reverse Transcription Polymerase Chain Reaction, Positive Control